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Par j 1.0101 is one of the two major allergens of the Parietaria judaica (Pj) pollen, and its three-dimensional structure was built by three-dimensional structural homology modeling. If you decide to continue browsing we consider that you accept their use. Your team's Premium Access agreement is expiring soon. Careful quantitative analyses by spot hybridization to homologous probes demonstrate that no rDNA amplification occurs during Paracentrotus lividus oogenesis. Parietaria pollen allergens, An update on allergens - Parietaria pollen allergens, Enhanced hybridization labeling signals in Southern blotted DNAs fractionated with voltage gradient gel electrophoresis, Identification of an immunodominant IgE epitope of the Parietaria Judaica major allergen, Isolation and Characterization of Two cDNA Clones Coding for Isof orms of the Parietaria judaica Major Allergen Par j 1.0101, cDNA cloning, sequence analysis and allergological characterization of Par j 2.0101, a new major allergen of the Parietaria judaica pollen, cDNA Sequence Analysis and Expression of the Ribosomal Protein S24 during Oogenesis and Embryonic Development of the Sea UrchinParacentrotus lividus, Rapid DNA Elution Procedure from Agarose Gels, Isolation of proteins and nucleic acids by electrophoresis on disposable gel columns, A Downward Capillary Blotting Procedure from Hundred Base Pairs to Hundred Kilobases Nucleic Acids, cDNA cloning, expression and primary structure of Par j I, a major allergen of Parietaria judaica pollen, Simple and Inexpensive Dot-Blot Apparatus, Sequence analysis of the rDNA spacer of Paracentrotus lividus and observations about pre-rRNA processing. Sequence analysis showed open reading frames of 176 and 138 amino acids. The cytotoxic effect of several diorganotin(IV) and triorganotin(IV)-meso-tetra(4-sulfonatophenyl)porphine derivatives was tested and only the (Bu(2)Sn)(2)TPPS and the (Bu(3)Sn)(4)TPPS showed cytotoxicity on A375 human melanoma cells. {{familyColorButtonText(colorFamily.name)}}, View {{carousel.total_number_of_results}} results. Since the biological functions of macromolecules are strongly correlated with their conformation, an effect on functional properties is also expected. The expressed recombinant protein represented the maj... We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchin Paracentrotus lividus. Sequence analysis shows a high percentage (90%) identity with the corresponding gene of Strongylocentrotus purpuratus. So-called stacking forces, of particular interest in the structural stability of nucleic acids, have been studied in a simple model system of planar, non-hydrogen-bonding dye molecules (Methylene Blue). We report on experiments showing that the presence of some monohydric alcohols in the solution medium perturbs the kinetics of bovine liver β-galactosidase. A spectrophotometric study of thionine dimerization in H2O and D2O, Effects of solvent on stacking interactions. Only verified researchers can join ResearchGate and send messages to other members. Starting from a crude extract of E. coli, the laboratory-made devise allowed the isolation of the maltose binding protein (MBP) fused to a re... A 659 bp cDNA clone** coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. Join Facebook to connect with Vincenzo Izzo and others you may know. the sea urchinP. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, subm... By the use of labeled DNA probes which selectively hybridize to non mature ribosomal RNA precursors and by the combined use Join ResearchGate to find the people and research you need to help your work. Western blot analysis of gene fragmentation products showed that the 1 to 30 region was capable... Two cDNA clones named P9* and P1* of 794 and 631 bp, respectively, were isolated from a lambda ZAP cDNA expression library using Parietaria judaica (Pj) pollen-specific IgE antibodies from a pool of sera (n = 23) of patients allergic to Pj. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies. The clone contained an insert of 622 nucleotides with an open reading frame of 133 amino acids (aa) and a putative signal peptide of 31 aa giving a deduced mature... A gene for the Paracentrotus lividus ribosomal protein S24, called P1-S24, has been isolated and sequenced. This region is about 3.8 Kb long. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. Click here to request Getty Images Premium Access through IBM Creative Design Services. In the present work we studied the initial part of thermal denaturation curves of B. subtilis DNA in normal and heavy water, observing, by electron microscopy, the “opening” of DNA as a function of temperature. Boards are the best place to save images and video clips. Izzo Caffè was founded near the Vesuvius in 1979 by Mr. Vincenzo Izzo as an artisan-based coffee roastery, which have been striving with passion to produce real Neapolitan espresso for over 25 years. About. Twenty-nine single sera from Pj-allergic subjects were test... We built a modified version of voltage gradient gel electrophoresis system to correct distortions in nucleic acids electrophoretic migration patterns occurring at the edges of the gel when the original voltage gradient apparatus is used. After a careful selection made by our experts on place of origin, the raw coffee is dispatched to our factory.Here we take some samples and we test them:- chemical analysis- samplingWhen these tests are passed, the roasting starts, during this phase the coffee is roasted at 200-240 degrees in order to gain the typical brown colour. Due to our privacy policy, only current members can send messages to people on ResearchGate. Par j 2.0101, a major allergen of the Parietaria judaica pollen, was expressed in E. coli, purified to homogeneity and fully characterised both at the structural and the functional level. © 2008-2020 ResearchGate GmbH. 36. A spectrophotometric study of methylene blue dimerization in aqueous solutions of some monohydric alcohols, Solvent isotope effect in the monomer?dimer equilibrium of methylene blue, Effects of Organic Solvents on the Conformational and Functional Stability of Some Biological Macromolecules, Effect of some monohydric alcohols on the functional stability of bovine liver ?-galactosidase, Thermal denaturation of B. subtilis DNA in H 2 O and D 2 O observed by electron microscopy, 266 Comparison of Allergen-Induced Late Inflammatory Reactions in the Nose and in the Skin in House Dust Mite-Allergic Patients with or without Asthma. Sorry, you need to be a researcher to join ResearchGate. This site uses cookies to improve services and experience of readers. The resultant model was used to identify putative IgE binding regions. All rights reserved. A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Contact. Too many images selected. Among the services the customers can benefit from, the Company have recently devised a formula for the franchised Izzo Coffee Shops, in cooperation with their business partners; cafeterias to which a series of promotional and commercial initiatives based on exclusive Izzo Products are bound. To examine the pathway of (Bu(2)Sn)(2)TPPS or (Bu(3)Sn)(4)TPPS induced A375 cell death, DNA fragmentation analysis,... An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Publications 36. NTS sequence of Paracentrotus lividus rDNA, Methods for recovering nucleic acid fragments from agarose gels, Voltage Gradient Electrophoresis of Nucleic Acids on Agarose Gels, Regulation of ribosomal RNA synthesis in sea urchin embryos, A method for eluting DNA in a wide range of molecular weights from agarose gels, Polymorphisms in the intergenic region of the sea urchin Paracentrotus lividus ribosomal DNA, Readthrough transcription occurs at the rho dependent signal F1 T IV in suppressor cells, Intragenomic recombination between homologous regions of genes II and IV promotes formation of bacteriophage f1 miniphages, Effects of solvent on stacking interactions. The new device allows correct fractionation of nucleic acids also when electrophoresis is performed at high volt... An enhancement of hybridization labeling signals is demonstrated in Southern blotted DNAs, fractionated by voltage gradient gel electrophoresis. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S... Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. Species belonging to this genus induce IgE responses in approximately 10 million people. Today the Company are offering a series of activities related to each other, which pursue the Company's aim to provide the market not only with a wide range of products but also with services tending to guarantee maximum customer satisfaction. Caffè Izzo awarded COFFEE OF THE YEAR 2016 Izzo Caffè - Our history . results support the hypothesis that D2O plays a stabilizing role on strands separation during thermal denaturation of DNA. View Vincenzo Izzo’s profile on LinkedIn, the world's largest professional community. The Vincenzo Catania, marito di Rossella Izzo: ecco come hanno cresciuto la figlia Myriam Rossella Izzo. Network. The dimerization process has been studied by spectral analyses at different concentrations and temperatures in D2O as well as in H2O. The same approach was used to measure the copy number of the genes involved in ribosome biogenesis. Surprisingly, differently from the organisms in which the lack of rDNA amplification phenom... A new device, based on the principle of voltage-gradient gel electrophoresis, was developed in order to enhance differentiation of the distance across the range of molecular masses in the electrophoretic fractionation of nucleic acids in an agarose matrix. lividus is very low in the pre-blastular stages and is m... We have developed a simple and rapid method for recovering DNAs of a wide range of molecular weights from agarose gels. The recombinant rPar j 2.0101 protein showed an allergenic activity in histamine release, skin prick tests and capacity to bind IgE, almost identical to that of t... Parietaria is a genus of dicotyledonous weeds of the Urticaceae family including several species and its pollen grain is one of the most important allergenic sources in the Mediterranean area. Publications. Il clan degli Izzo, come raccontato anche dalla gieffina Myriam Catania, è molto unito; una grande famiglia che ama riunirsi e passare del tempo insieme. This enhancement is due to a reduced thickness of each single nucleic acid band in the gel as a consequence of the gradient effect, corresponding to an increased concentration of DNA per unit area. The IBM strategic repository for digital assets such as images and videos is located at dam.ibm.com. of Northern blots and ofin vivo labeling experiments we provide evidence for the following facts: 1) the rate of rRNA synthesis per embryonic nucleus in {{collectionsDisplayName(searchView.appliedFilters)}}, {{searchText.groupByEventToggleImages()}}, {{searchText.groupByEventToggleEvents()}}. Reads . A disposable syringe was modified to host a gel column and an elution chamber. Find the perfect Vincenzo Izzo stock photos and editorial news pictures from Getty Images. Both clones contain a putative signa... A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using a pool of 23 sera from Pj-allergic patients. View the profiles of people named Vincenzo Izzo. Results of a spectrophotometric study at different temperatures of the monomerdimer equilibrium of thionine solutions in H2O and in D2O confirm and extend previous findings, obtained by studying the self-association of Methylene Blue in aqueous solutions, concerning the involvement of solvent rearrangement processes in the stacking of planar dye mo... A previous experimental study of the role of hydrophobic interactions on so-called stacking forces (of considerable interest in the conformational stability of nucleic acids) is here extended by considering alcohol solvent perturbation, as probed by dye self-association. Hybridization of the cDNA to dige... A simple and cheap one-step method to isolate proteins or nucleic acids by electrophoresis in disposable gel columns is reported. 1,278. Select 100 images or less to download. Vincenzo has 8 jobs listed on their profile. The number of rDNA repeat subclasses distinguishable within any individual sea urchin is usually limited (1 to 3) with respect to the wi... Suppressor cells Infected with bacteriophage f1 yield phage encoded gene IV transcripts longer than those present in the sup° host and identical to those found in a rho- host. In this paper the use of voltage gradient gel electrophoresis (VGGE) in the electrophoretic analysis of apoptotic DNAs is described. The Getty Images design is a trademark of Getty Images. See the complete profile on LinkedIn and discover Vincenzo’s connections and jobs at similar companies. © 2020 Getty Images. GRUPPO IZZO Srl - Italy - 03012 Anagni (FR) Via Morolense, Km 2 Tel +39 0775 774036 - Fax +39 0775 768524 - P.IVA 03043961212 - Credits. The peculiarity of VGGE fractionation in enhancing DNA bands in the gel by reducing their thickness was used to obtain a rapid, more selective and higher-quality electrophoretic fractionation of apoptotic DNA with res... Pollen allergens are multivalent proteins that cross-link IgE antibodies on mast or basophil cells, inducing secretion of biologic mediators, and resulting in various allergic symptoms. Vincenzo Izzo. About. The IgE-binding regions of the Parietaria judaica (Pj) pollen major allergen rPar j 2 were investigated. The effect increases with increasing alcohol concentration and alkyl group size. Aware of the progresses achieved so far, and steadily committed to continuous and constant improvement, we pride ourselves today on playing an important role in the complex scenario of coffee business, especially thanks to the dynamism of our ideas. A DNA-containing gel slice is placed on a Parafilm sheet in the center of a circular (positive) electrode and covered with a drop of buffer, while a linear (negative) electrode is placed on the top of the gel and driven about 1 mm... Blot-hybridizations of the sea urchin Paracentrotus lividus genomic DNA with ribosomal DNA (rDNA) probes revealed individual variations in the length and in the sequence of the non-transcribed spacer (NTS) region. University students and faculty, institute members, and independent researchers, Technology or product developers, R&D specialists, and government or NGO employees in scientific roles, Health care professionals, including clinical researchers, Journalists, citizen scientists, or anyone interested in reading and discovering research. 29.57. Diorganotin(IV) and triorganotin(IV) complexes of meso-tetra(4-sulfonatophenyl)porphine induce apoptosis in A375 human melanoma cells, Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels, Assignment of Disulphide Bridges in Par j 2.0101, a Major Allergen of Parietaria judaica Pollen, Lack of amplification phenomena of ribosomal RNA genes in sea urchin, Multiple voltage‐gradient gel electrophoresis system, Use of voltage gradient gel electrophoresis in apoptotic DNA analysis, The IgE-binding epitopes of rPar j 2, a major allergen of Parietaria judaica pollen, are heterogeneously recognized among allergic subjects, Modified apparatus for voltage gradient gel electrophoresis, An update on allergens. Collect, curate and comment on your files. In the course of time, the strong spirit of enterprise supported by a highly qualified staff, both in the technical and commercial field, have favoured the rapid development of the Company and the establishment of its presence on both the national and international market. Biography. This repository is populated with tens of thousands of assets and should be your first stop for asset selection. Ribosomal protein P1-S24 consists 130 amino acids and has a molecular weight of 14869 Da. Rossella Izzo: Relatives: Renato Izzo (grandfather) Simona Izzo (aunt) Fiamma Izzo (aunt) Giuppy Izzo (aunt) Francesco Venditti (cousin) Myriam Catania (born December 12, 1979) is an Italian actress and voice actress. Computer-aide... As it is well known, solvent perturbation due to the presence of organic solvent in the solution medium affects the conformational equilibria of biological macromolecules1,2,3. Izzo Caffè was founded near the Vesuvius in 1979 by Mr. Vincenzo Izzo as an artisan-based coffee roastery, which have been striving with passion to produce real Neapolitan espresso for over 25 years. Select from premium Vincenzo Izzo of the highest quality. Although several methods for recovering DNA and RNA molecules hav... A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about 30 kb) in the same electrophoresis agarose gel.

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